2024
Siu-Shing Wong, Alan Wainman, Saroj Saurya, Jordan W Raff. Regulation of centrosome size by the cell-cycle oscillator in Drosophila embryos. EMBO J 2024 Jan 17 |PDF|
2023
Zachary M. Wilmott and Jordan W. Raff. A simple Turing reaction-diffusion model explains how PLK4 breaks symmetry during centriole duplication and assembly. PLOS Biology 2023 Nov 20 |PDF|
2022
Zachary M. Wilmott and Jordan W. Raff. Cell cycle: Making waves to coordinate the entry into mitosis. Current Biology 2022 Nov 21 |PDF|
Thomas L. Steinacker, Siu-Shing Wong, Zsofia A. Novak, Saroj Saurya, Lisa Gartenmann, Eline J.H. van Houtum, Judith R. Sayers, B. Christoffer Lagerholm, and Jordan W. Raff. Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL. J Cell Biol 2022 Sep 5 |PDF|
Mustafa G. Aydogan, Laura E. Hankins, Thomas L. Steinacker, Mohammad Mofatteh, Saroj Saurya, Alan Wainman, Siu-Shing Wong, Xin Lu, Felix Y. Zhou and Jordan W. Raff. Centriole distal-end proteins CP110 and Cep97 influence centriole cartwheel growth at the proximal-end. J Cell Sci 2021 June 22. |PDF|
Siu-Shing Wong, Zachary M Wilmott, Saroj Saurya, Ines Alvarez-Rodrigo, Felix Y Zhou, Kwai-Yin Chau, Alain Goriely, & Jordan W Raff. Centrioles generate a local pulse of Polo/PLK1 activity to initiate mitotic centrosome assembly. EMBO J2022, 3 May. |PDF|
2021
Ines Alvarez-Rodrigo, Alan Wainman, Saroj Saurya and Jordan W. Raff. Ana1/Cep295 helps recruit Polo/PLK1 to centrioles to promote mitotic PCM assembly and centriole elongation. J Cell Sci 2021 June 22. |PDF|
2020
Mustafa G. Aydogan, Thomas L. Steinacker, Mohammad Mofatteh, Zachary M. Wilmott, Felix Y. Zhou, Lisa Gartenmann, Alan Wainman, Saroj Saurya, Zsofia A. Novak, Siu-Shing Wong, Alain Goriely, Michael A. Boemo, Jordan W. Raff. An Autonomous Oscillation Times and Executes Centriole Biogenesis. Cell 181, 1-16, 2020. |PDF|
Lisa Gartenmann, Catarina C. Vicente, Alan Wainman, Zsofi A. Novak, Boris Sieber, Jennifer H. Richens, Jordan W. Raff. Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole/centrosome assembly. J Cell Sci 2020 May 14. |PDF|
Alan Wainman. Expansion microscopy on Drosophila spermatocyte centrioles. Methods in Cell Biology Jul 18, 2020 | PDF|
2019
Ines Alvarez-Rodrigo, Thomas L Steinacker, Saroj Saurya, Paul T Conduit, Janina Baumbach, Zsofia A Novak, Mustafa G Aydogan, Alan Wainman, Jordan W Raff. Evidence that a positive feedback loop drives centrosome maturation in fly embryos. eLife. 2019 September 9. |PDF|
Jordan W. Raff. Phase Separation and the Centrosome: A Fait Accompli? Trends Cell Biol. 2019 May 7. |PDF|
2018
Mustafa G. Aydogan, Alan Wainman, Saroj Saurya, Thomas L. Steinacker, Anna Caballe, Zsofia A. Novak, Janina Baumbach, Nadine Muschalik, and Jordan W. Raff. A homeostatic clock sets daughter centriole size in flies Journal of Cell Biology March 2, 2018. |PDF|
Helio Roque, Saroj Saurya, Metta B. Pratt, Errin Johnson, Jordan W. Raff. Drosophila PLP assembles pericentriolar clouds that promote centriole stability, cohesion and MT nucleation. PLoS Genetics 14(2): e1007198, 2018. |PDF|
2017
Lisa Gartenmann, Alan Wainman, Maryam Qurashi, Rainer Kaufmann, Sebastian Schubert, Jordan W. Raff, Ian M. Dobbie. A combined 3D-SIM/SMLM approach allows centriole proteins to be localized with a precision of ∼4–5 nm. Current Biology 27, Issue 19, pR1054–R1055, 2017 |PDF|
Zhe Feng, Anna Caballe, Alan Wainman, Steven Johnson, Andreas F.M. Haensele, Matthew A. Cottee, Paul T. Conduit, Susan M. Lea, Jordan W. Raff. Structural Basis for Mitotic Centrosome Assembly in Flies. Cell 169, 1078–1089, 2017 |PDF|
Cottee MA, Johnson S, Raff JW, Lea SM. A key centriole assembly interaction interface between human Plk4 and STIL appears to not be conserved in flies. Biology Open 2017: |PDF|
2016
Metta B. Pratt, Joshua S. Titlow, Ilan Davis, Amy R. Barker, Helen R. Dawe, Jordan W. Raff and Helio Roque. Drosophila sensory cilia lacking MKS-proteins exhibit striking defects in development but only subtle defects in adults. J Cell Sci 2016: |PDF|
Zsofia A. Novak, Alan Wainman, Lisa Gartenmann, Jordan W. Raff. Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes. Dev Cell Volume 37, Issue 6, p545–557, 20 June 2016. |PDF|
Saroj Saurya, Helio Roque, Zsofia A. Novak, Alan Wainman, Mustafa G. Aydogan, Adam Volanakis, Boris Sieber, David Miguel Susano Pinto, and Jordan W. Raff. Drosophila Ana1 is required for centrosome assembly and centriole elongation. J Cell Sci 2016 : doi: 10.1242/jcs.186460 |PDF|
2015
Paul T Conduit, Alan Wainman, Zsofia A Novak, Timothy T Weil, Jordan W Raff. Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP. eLife 2015;4:e08483 |PDF|
Conduit PT, Wainman A, Raff JW. Centrosome function and assembly in animal cells. Nat Rev Mol Cell Biol. |PDF|
Paul T. Conduit and Jordan W. Raff. Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. Current Biology 25, R635–R653. |PDF|
Jennifer H. Richens, Teresa P. Barros, Eliana P. Lucas, Nina Peel, David Miguel Susano Pinto, Alan Wainman and Jordan W. Raff. The Drosophila Pericentrin-like-protein (PLP) cooperates with Cnn to maintain the integrity of the outer PCM. Biology Open |PDF|
Matthew A Cottee, Nadine Muschalik, Steven Johnson, Joanna Leveson, Jordan W Raff, Susan M Lea. The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. eLife |PDF|
Janina Baumbach, Zsofia Anna Novak, Jordan W. Raff and Alan Wainman. Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo. PLoS Genetics 11(5): e1005261 |PDF|
2014
Paul T. Conduit, Jennifer H. Richens, Alan Wainman, James Holder, Catarina C. Vicente, Metta B. Pratt, Carly I. Dix, Zsofia A. Novak, Ian M. Dobbie, Lothar Schermelleh and Jordan W. Raff. A molecular mechanism of mitotic centrosome assembly in Drosophila. eLife |PDF|
Zsofia A. Novak, Paul T. Conduit, Alan Wainman, Jordan W. Raff. Asterless Licenses Daughter Centrioles to Duplicate for the First Time in Drosophila Embryos. Current Biology June 2, 2014. 24, 1-7 |PDF|
Conduit, P.T., Feng, Z., Richens, J.H., Baumbach, J., Wainman, A., Bakshi, S., Dobbelaere, J., Johnson, S., Lea, S.M., and Raff, J.W. The centrosome specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation. Dev. Cell 2014 28 659-669|PDF|
2013
Cottee M.A., Muschalik N., Wong Y.L., Johnson C.M., Johnson S., Andreeva A., Oegema K., Lea S.M., Raff J.W., van Breugel M. Crystal structures of the CPAP/STIL complex reveal its role in centriole assembly and human microcephaly. eLife 2013 2, e01071|PDF|
Franz, A., Roque, H., Saurya, S., Dobbelaere, J., and Raff, J.W. Novel functions for Drosophila CP110 in regulating centriole assembly in vivo. J. Cell Biol. 2013 203 785-99|PDF|
Conduit P.T. The dominant force of Centrobin in centrosome asymmetry. Nat. Cell. Biol. 2013 15 (3) 235-237|PDF|
Watts, C.A., Richards, F.M., Bender, A., Bond, P.J., Korb, O., Kern, O., Riddick, M., Owen, P., Myers, R.M., Gergely, F., Raff, J.W., Jodrell, D.I., and Ley, S.V. Design, synthesis and biological evaluation of an allosteric inhibitor of HSET that targets cancer cells with supernumerary centrosomes. Chemistry and Biology 2013 20 1-12|PDF|
2012
Roque, H., Wainman, A., Richens, J., Kozyrska, K., Franz, A., Raff, JW. Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. J. Cell Sci. 2012 125 5881-5886|PDF|
Baumbach, J., Levesque, MP., Raff, JW. Centrosome loss or amplification does not dramatically perturb global gene expression in Drosophila. Bio. Open 2012 doi: 10.1242/bio.20122238 |PDF|
Reschen, RF., Colombie, N., Wheatley, L., Dobbelaere, J., St Johnston, D., Ohkura, H., Raff, JW. Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC. Bio. Open 2012 1(5) 422-429 |PDF|
2011
Cottee, MA., Raff, JW., Lea, SM., and Roque, H. 2011. SAS-6 oligomerization: the key to the centriole? Nat. Chem. Biol. 7(10) 650-653 |PDF|
2010
Stevens, N., Dobbelaere, J., Brunk, K., Franz, A., and Raff, JW. 2010. Drosophila Ana2 is a conserved centriole duplication factor JCB. 188(3) 313-323 |PDF|
Stevens, N., Roque, H., and Raff, JW. 2010. DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement Dev. Cell 19(6) 913 – 919 |PDF|
Conduit, PT., Brunk, K., Dobbelaere, J., Dix, CI., Lucas, EP., Raff, JW. 2010. Centrioles regulate centrosome size by controlling the rate of Cnn incorporation into the PCM Curr. Biol. 20(24) 2178 – 2186 |PDF|
Conduit, PT., and Raff, JW. 2010. Cnn dynamics drive centrosome size asymmetry to ensure daughter centriole retention in drosophila neuroblasts Curr. Biol. 20(24) 2187 – 2192 |PDF|
2009
Stevens, N., Dobbelaere, J., Wainman, A., Gergely, F., Raff, JW. 2009. Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies. JCB. 187(3) 355-363|PDF|
Nigg, E and Raff, JW 2009. Centrioles, Centrosomes, and Cilia in Health and Disease. Cell. 139(4) pp. 663 – 678. |PDF|.
2008
Dobbelaere, J., Josue, F., Suijkerbuijk, S., Baum, B., Tapon, N., and Raff, J.W. 2008. A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila. PloS Biology. 6, e224. |PDF|
Basto, R., Brunk, K., Vinadogrova, T., Peel, N., Franz, A., Khodjakov, A., and Raff, J.W. 2008. Centrosome amplification can initiate tumourigenesis in flies. Cell. 133, 1032-1042. |PDF|.
2007
Dix, C., and Raff, J.W. 2007. Drosophila Spd-2 is essential for PCM recruitment to the sperm centriole, but not for centriole duplication. Curr. Biol. 17, 1759-64.|PDF|
Stevens, N.R., Raposo, A.S.F., Basto, R., St Johnston, D., and Raff, J.W. 2007. From stem cell to embryo without centrioles. Curr. Biol. 21, 1498-1503. |PDF|
Lucas, E., and Raff, J.W. 2007. Maintaining the proper connection between the centrioles and the PCM requires Drosophila Centrosomin. J. Cell Biol. 178, 725-732.|PDF|
Peel, N., Stevens, N.R., Basto, R., and Raff, J.W. 2007. Overexpressing centriole replication proteins in vivo induces centriole overduplication and de novo formation. Curr. Biol. 17, 834-843.|PDF|
Basto, R., Gergely, F., Draviam, V.M., Ohkura, H., Liley, K., Raff, J.W. 2007. Hsp90 is required to localize Cyclin B and Msps/Ch-Tog to the mitotic spindle in Drosophila and humans. J. Cell Sci. 120, 1278-1287.|PDF|
2006
Basto, R., Lau, J., Vinogradova, T., Gardiol, A., Wood, G., Khodhjakov, A., and Raff, J.W. 2006. Flies without centrioles. Cell 125, 1375-1386.|PDF|
2005
Barros, T., Kinoshita K., Hyman, A.A., and Raff, J.W. 2005. Aurora-A activates D-TACC/Msps complexes exclusively at centrosomes to stabilise centrosomal MTs. J. Cell. Biol. 170, 1039-1046.|PDF|
Kinoshita, K., Noetzel, T.L., Pelletier, L., Mechtler, K., Drechsel, D.N., Schwater, A., Lee, M., Raff, J.W., and Hyman, A.A. 2005. Aurora A phosphorylation of TACC3/Maskin is required for centrosome dependent microtubule assembly in mitosis. J. Cell Biol. 170, 1047-1055.|PDF|
Chodagam, S., Royou, A., Whitfield, W., Karess, R., and Raff J.W. 2005. The centrosomal protein CP190 regulates myosin function during early Drosophila development. Curr. Biol. 15, 1308-1313.|PDF|
2004
Martinez-Campos, M., Basto, R., Baker, J., Kernan, M., and Raff., J.W. 2004. The Drosophila pericentrin-like protein is essential for cilia/flagella function but appears to be dispensable for mitosis. J. Cell Biol.165, 673-683.|PDF|
Raff, J. W. 2004. Centrosomes in a developing organism: lessons from Drosophila. In: “Centrosomes in Development and Disease”. Ed. Nigg, E.A. Wiley-VCH.
Butcher, R.D.J., Chodagam, S., Basto, R., Wakefield, J., Henderson, D.S., Raff, J.W., and Whitfield, W.G.F. 2004. The Drosophila centrosome-associated protein CP190 is essential for viability, but not for cell division J. Cell Sci. 117, 1191-1199.|PDF|
2003
Raff, J. W. 2003. Genome maintenance. Nature. 423, 493-495. |PDF|
Gergely, F., Mythily, V., Lee, M., and Raff, J.W. 2003. The ch-Tog/XMAP215 protein is essential for spindle pole organisation in human somatic cells. Genes and Dev. 17, 336-341. |PDF|
2002
Huang, J-Y., and Raff, J.W. 2002. The dynamic localisation of the Drosophila APC/C: evidence for the existence of multiple complexes that perform distinct functions and are differentially localised. J. Cell Sci.115, 2847-2856.|PDF|
Raff, J.W., Jeffers, K., and Huang, J-Y. 2002. The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time. J. Cell Biol. 157, 1139-1149.|PDF|
Raff, J.W. 2002. Centrosomes and Cancer: lessons from a TACC. Trends Cell Biol. 12, 222-225. |PDF|
2001
Lee, M.J., Gergely, F., Jeffers, K., Peak-Chew, S-Y., and Raff, J.W. 2001. Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour. Nat. Cell Biol.. 3, 643 – 649.|PDF|
Raff, J.W. 2001. Centrosomes: central no more? Current Biology. 11, R159-R161. |PDF|
2000
Gergely, F., Karlsson, C., Still, I., Cowell, J., Kilmartin, J., and Raff, J.W. 2000. The TACC domain identifies a new family of centrosomal proteins that can interact with microtubules. Proc. Nat. Acad. Sci.97, 14352-57.|PDF|
Wakefield, J.G., Huang, J-Y., and Raff, J.W. 2000. A role for centrosomes in regulating the destruction of cyclin B in early Drosophila embryos. Current Biology. 10, 1367-1370.|PDF|
Gergely, F., Kidd, D., Jeffers, K., Wakefield, J.G., and Raff, J.W. 2000. D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo. EMBO J. 19, 241-252.|PDF|